Catherine LEVISAGE

To reproduce the complex 3 dimensional (3D) microenvironment experienced by cells in vivo, we have selected hydrogels, i.e complex networks of hydrophilic crosslinked polymers, for their similarity with the extracellular matrix of tissues. Our research is focused on natural, bioresorbable and non-immunogenic polysaccharides that are already commercialized for medical applications. We investigate hydroxypropyl methylcellulose (cotton) and pullulan (yeast fermentation) to design innovative hydrogels with tunable porosity and network diffusion parameters. Using various fabrication techniques, we develop chemically cross-linked hydrogels with complex architectures, tunable viscoelasticity and desired ability to mediate cellular activities through the spatial and temporal presentation of biological cues. We investigate hydrogels as i) tools for stem cell-based organogenesis and ii) carriers of cells or bioactive molecules for bio-inspired delivery strategies in cartilage and intervertebral disc regeneration.

Recent publications